12th International Symposium on Bioluminescence & Chemiluminescence

Symposium abstracts:

Development of allergology test in microplate immunoassay format based on chemiluminescence

Pazzagli, Mario¹, Pinzani, Pamela¹, Manzoni, Angelo², Salti, Sara², Passeri, Laura²

1. Dept of Clinical Physiopathology, Clinical Biochemistry Unit, University of Florence, Italy. Email: m.pazzagli@dfc.unifi.it

2. Radim, R&D Department, Via del Mare,125, 00040 Pomezia Rome, Italy. Email: l.passeri@radim.it

Lumigen PS-1 affords numerous advantages: the higher peak chemiluminescence intensities, longer signal duration and extended linearity achieved compared to other detection reagents suggested that its use may lead to improvements in detecting HRP conjugates in immunoassay as well. In order to study the real advantages which can be obtained with luminescence detection instead of photometry in microplate format assays, a comparative study was performed using reagents produced by RADIM for CARLA® (Capture Assay Radim Liquid Alleergen) Allergy System with colorimetric end-point and the modified format which include white coated microplates and Lumigen PS-1 as the substrate.The chemiluminescent endpoint allowed a reduction of incubation time from 90 to 60 minutes and sample volume from 100 to 50 microlitres (paediatric sample), extended linearity (sensitivity and dosages from classes to concentration), improved specificity at low concentration. These characteristics make the application suitable for the development of an automatic analytical system for fast patient allergy profile test based on microplate based on microplate platform

This is a preprint of an article accepted for publication in Luminescence: Copyright 2001 John
Wiley & Sons, Ltd (Wiley website)