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12th International Symposium on Bioluminescence & Chemiluminescence |
Symposium abstracts:
Nofer, L., Berthold, A.
Berthold Detection Systems GmbH, Bleichstrasse 56-68, 75173 Pforzheim Germany
Since luminometers have found wide acceptance in the scientific, industrial and in the clinical diagnostic community, users have asked for reliable and user-friendly devices to validate their instruments. For tube luminometers, radioactive sources, sealed with scintillating material have been available, emitting light with a given intensity and adequate stability. These sources will cover just one intensity, though. For microplate luminometers, however, no such devices have found wide acceptance so far. There are a number of reasons for this, both technical and commercial (excessive manufacturing cost). Berthold Detection Systems developed a new test plate with the following features: non-radioactive, light emission by light-emitting diodes (LEDs) of defined emission wavelength, battery operated, therefore instrument-independent, 14 individual measurement points of varying, but very stable intensity, spread of 6 orders of magnitude. Prototypes have been monitored for two years, finished products have been monitored for one year. They all show satisfactory stability. The product works in a large number of commercially available luminometers, but also with camera-based imaging systems. The technical features will be described in more detail. A comparative analysis with test plates using different technologies will be presented.
This
is a preprint of an article accepted for publication in Luminescence: Copyright
2001 John
Wiley & Sons, Ltd (Wiley website)