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12th International Symposium on Bioluminescence & Chemiluminescence |
Symposium abstracts:
Francis, Paul S.1, Lewis, Simon W.1, Lim, Kieran F.1, Jenkins, Graeme E.2
1. Centre for Chiral and Molecular Technologies, School of Biological and Chemical Sciences, Deakin University, Geelong, Victoria 3217, Australia
2. Precision Devices P/L, 44 Nelson Street, Shoreham, Victoria, 3916, Australia
We have investigated the emission of light accompanying the reaction between urea and hypobromite [1] with the intention of establishing the true light-producing pathway and the structural requirements for an intense emission. The knowledge gained in these investigations has assisted the analytical application of this reaction [2], and we have demonstrated its use for the continuous monitoring of haemodialysis treatments given to patients with chronic renal failure [3].
A key component of this research has been the development of novel sample handling instrumentation based on a pulsed solution flow [4]. This robust and potentially portable instrumentation, coupled with rapid and selective chemiluminescence detection, is particularly attractive for fields such as clinical diagnostics, process analysis, environmental monitoring and the forensic sciences . In addition to the analytical applications of these devices, the ability to generate emission-time profiles has provided data on reaction kinetics that has supplemented our spectroscopic studies in the pursuit of the light-producing pathway.
1. J. Stauff and H. Schmidkunz, Z. Phys. Chem., Neue Folge, 33 (1962) 273. 2. P. S. Francis, S. W. Lewis and K. F. Lim (2001) Trends. Anal. Chem., In press. 3. S.W. Lewis, P.S. Francis, K.F. Lim and G.E. Jenkins (2001) Submitted for publication. 4. S.W. Lewis, P.S. Francis, K.F. Lim, G.E. Jenkins and X.D. Wang (2000) Analyst, 125, 1869
This
is a preprint of an article accepted for publication in Luminescence: Copyright
2001 John
Wiley & Sons, Ltd (Wiley website)