12th International Symposium on Bioluminescence & Chemiluminescence

Symposium abstracts:

Enzyme kinetics model of the bacterial luciferase reactions for biosensor application

Feng, Yi ¹, Davies, Rupert H.², Andrade, Joseph D.^{1, 2}

Dept of Materials Science ¹ and Bioengineering ²,University of Utah, Salt lake City, Utah 84112, USA

E-mail: yf3@utah.edu, rupert.davies@m.cc.utah.edu, joeandrade@uofu.net

Reduced Nicotinamide Adenine Dinucleotide (NADH)-based bioluminescence assays are being employed in multi-analyte biosensors. Our NADH sensing platform involves NADH-flavin mononucleotide (FMN) oxidoreductase (Vibrio fischeri) and bacterial luciferase (Vibrio harveyi). The biochemical mechanisms of these reactions have been studied and models have been proposed. As our goal is focused on biosensor engineering, we have simplified these complex kinetic models while retaining their usefulness for biosensor design and optimization. The model is applied to the measurement of NADH and to the measurement of lactate and phenylalanine via the dehydrogenase pathways.

This is a preprint of an article accepted for publication in Luminescence: Copyright 2001 John
Wiley & Sons, Ltd (Wiley website)