12th International Symposium on Bioluminescence & Chemiluminescence

Symposium abstracts:

Direct immunodetection of proteins within precast polyacrylamide gels

Desai, Surbhi, Dworecki, Boguslawa, Cichon, Eugene

Pierce Chemical Company, Rockford, Illinois 61105, USA

Chemiluminescent detection of specific proteins separated by SDS-PAGE is the basis for studying specific antigens. Chemiluminescent immunodetection if antigens is most commonly performed using Western blotting technique. We have shown that it is possible to eliminate Western blotting and to directly detect the antigens within precast polyacrylamide gels. By combining cutting edge enhanced chemiluminescence with an alcohol pretreatment of the gel, sensitivity comparable to standard enhanced chemiluminescent detection can be achieved. This method is extremely valuable for large or difficult to transfer proteins.

This is a preprint of an article accepted for publication in Luminescence: Copyright 2001 John
Wiley & Sons, Ltd (Wiley website)