12th International Symposium on Bioluminescence & Chemiluminescence

Symposium abstracts:

Application of luciferase transfected human tumor cell lines for chemotherapeutic drug development

Andreotti, Peter E.¹, Caceras, Gisela², Zamkina, Ralitza², Escano, Maria C.², Price, Victoria¹, Dauphinee, Michael²

1. ASD Inc., PMB 46, 1511 E. Commercial Blvd, Fort Lauderdale, Florida 33334, USA
2. Rumbaugh Goodwin Institute For Cancer Research, Plantation, Florida 33313, USA

Human breast and prostate tumor cell lines transfected with Photinus pyralis luciferase (luc) gene have been used for in vitro chemotherapeutic drug development studies. In vitro systems using the transfected tumor cell lines provide a sensitive, rapid, and simple method for screening and testing agents with robot compatible microplate luminometers. The reporter cell lines have been used in vitro to study the kinetics, dose-dependence, and combination effects for a variety of agents including new and established chemotherapeutic drugs, monoclonal antibodies, and peptides. Results will be presented using the transfected tumor cell lines to test different chemotherapeutic agents compared to multi-step assay methods requiring ATP extraction and addition of exogenous luciferase.

This is a preprint of an article accepted for publication in Luminescence: Copyright 2001 John
Wiley & Sons, Ltd (Wiley website)