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12th International Symposium on Bioluminescence & Chemiluminescence |
Symposium abstracts:
Rubtsova, Maya Yu., Samsonova, Zhanna V., Ezhov, Alexander A., Egorov,
Alexey M.
Dept of Chem Enzymology, Chemistry Faculty, M.V. Lomonosov Moscow State University,
119899 Moscow, Russia. Email: myr@enz.chem.msu.ru
Chemiluminescent dipstick tests with a multispot membrane strip suitable for the on-site detection of several pesticides in water have been evaluated. Our approach for the multianalyte assay consisted in the simultaneous covalent immobilization of antibodies with different specificity towards pesticides (2,4-D and s-triazines) as discrete zones on the membrane strip. Membrane-based immunoassay technique with horseradish peroxidase as a label was combined with a sensitive and fast enhanced chemiluminescent (ECL) detection on a portable luminometer. The dipstick tests developed allow the determination of pesticides in water at concentrations above 0.02 µg/L. The first test discriminates the pesticides from two different groups (2,4-D and s-triazines) at the threshold value of 0.1µg/L. The second test was combined with artificial neural network in order to identify the cross-reacting s-triazines (atrazine, terbuthylazine and ametryn) in mixtures. The correct s-triazine classification of environmental samples is possible in 73-100% of all cases depending on the type of analyte.
This
is a preprint of an article accepted for publication in Luminescence: Copyright
2001 John
Wiley & Sons, Ltd (Wiley website)