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12th International Symposium on Bioluminescence & Chemiluminescence |
Symposium abstracts:
Kudryasheva, Nadezhda S., Nemtseva Elena V.1, Sizykh, Arnold G.2, Visser, A.J.W.G.3
1. Institute of Biophysics, Krasnoyarsk, 660036, Russia
2. Krasnoyarsk State University, Krasnoyarsk, 660061, Russia
3. MicroSpectroscopy Center, Department of Biomolecular Sciences, Wageningen
University, 6703 HA Wageningen, The Netherlands
The hypothesis on activity of upper electron-excited states of bacterial bioluminescent
emitter was verified with dye molecules as foreign energy acceptors. Spectra
of the bacterial bioluminescent coupled enzyme system NADH:FMN-oxidoreductase
- luciferase in the presence of these compounds was under investigation. Six
compounds were selected having the energy of the fluorescent states ranged from
25 700 to 32000 cm-1 (anthracene, pyrene, POPOP, MSB, 2-methoxy-naphtalene,
p-terphenyl), exceeding that of the bioluminescent emitter (22000 cm-1).
Their absorption spectra do not overlap with the bioluminescence spectrum; the
trivial light absorption and intermolecular resonance S-S transfer were excluded.
The weak sensitized fluorescence of foreign energy acceptor molecules was registered.
The results obtained have confirmed the activity of energetic precursor in bacterial
bioluminescence. Its energy can be localized about 26000 cm-1.
This
is a preprint of an article accepted for publication in Luminescence: Copyright
2001 John
Wiley & Sons, Ltd (Wiley website)