Symposium 1997: CV for Aladar A Szalay

Professor Aladar A Szalay

Address for correspondence: Aladar A. Szalay, PhD Professor/Associate Director, Center for Molecular Biology & Gene Therapy, Loma Linda University, California, USA
Biographical Sketch

Education
PhD Biochemistry-January 1972, Institute of Biochemistry, Halle/Saale, Germany
MS Biochemistry-July 1966, Martin Luther University, Halle/Saale, Germany

Research and/or professional experience
1971-1973: Senior Scientist, Biological Research Center, Hungarian Academy of Sciences
1973-1975: Research Associate California Institute of Technology
1975-1977: Senior Associate at California Institute of Technology
1977-1987: Assoc. Biochemist Group Leader: Plant Mole Bio, Boyce Thompson Institute
1977-1987: Professor, Division of Biological Sciences, Cornell Univ, Ithaca, New York Member of the Graduate Field Microbiology Member of the Graduate Field of Plant Molecular Biology
1987-1995: Professor & Chair of Plant Molecular Genetics; and Director of Agriculture Forestry Molecular Biology & Biotechnology Centre University of Alberta, Edmonton, Alberta Canada
1987-1995: Joint Appointments; Professor, Department of Anatomy and Cell Biology, Faculty of Medicine, Professor, Department of Genetics, Faculty of Science; Professor, Dept of Micro., Faculty of Science, Univ of Alberta, Edmonton
1991-present: Foreign Member of the Bio Research Ctr, Hungarian Academy of Sciences
1993-1996 Founding Director, Center for Molecular Biology & Gene Therapy, Loma Linda Univ. Loma Linda CA
1993 to present: Professor, Microbiology & Molecular Genetics Associate Director for Center for Molecular Biology & Gene Therapy, Loma Linda University, Loma Linda CA
1993 to present: Elected Member, Dept of Biochem., University of Georgia,Georgia, USA

Fellowships and awards

Wilhelm Exner Award - Vienna Austria 1993 (Medal for excellence in Science)
Distinguished lecturer for the Economic Council of Advanced Technology in Edmonton, 1989
Distinguished lecturer in Agricultural Biotechnology, University of Maryland, 1988
Alexander von Humboldt Scholar Award 1985, Max Planck Institute, Koln, Germany
Theobald Smith Lecturer 1985 awarded by Squibb, Merck Corp. and Princeton University
Gosney Fellowship 1973-1975, California Institute of Technology, Pasadena, CA
Graduate Student Fellowship in Biochemistry 1966-1971, Awarded by the Hungarian Nat'l Academy of Sciences

Selected publications related to visualization of light emission in living cells, from 1986

Legocki, R.P., Legocki, M., Baldwin, T.O., and Szalay, A.A. (1986)
Bioluminescence in soybean root nodules: Demonstration of a general approach to assay gene expression in vivo using bacterial luciferase. Proc. Natl. Acad. Sci. USA, 83:9080-9084
Koncz, C., Martini, N., Koncz-Kalman, Z., Olsson, O., Radermacher, A., Szalay, A., and Schell, J. (1987)
Genetic tools for the Analysis of Gene Expression in Plants. Basic Life Science, 41:197-209
Koncz, C., Olsson, O., Langridge, W.H.R., Schell, J., and Szalay, A.A. (1987)
Expression and assembly of functional bacterial luciferase in plants. Proc. Natl. Acad. Sci. USA, 84:131-135
O'Kane, D.J., Lingle, W.L., Wampler, J.E., Legocki, M., Legocki, R.P., and Szalay, A.A. (1988)
Visualization of bioluminescence as a marker of gene expression in Rhizobium-infected soybean root nodules. J. Plant Mol.Biol., 10:387-399
Olsson, O., Escher, A., Sandberg, G., Schell, J., Koncz, C., and Szalay, A.A. (1989)
Engineering of monomeric bacterial luciferase by fusion of lux A and lux B genes of Vibrio harveyi. Gene., 81:335-347
Olsson, O., Koncz, C., and Szalay, A. (1989)
The use of the luxA gene of the bacterial luciferase operon as a reporter gene. Mol. Gen. Genet., 215:1-9
Langridge, W.H.R., Fitzgerald, K.J., Koncz, C., Schell, J., and Szalay, A.A. (1989)
Dual promoter of Agrobacterium tumefaciens mannopine synthase genes is regulated by plant growth hormones. PNAS, 86:3219-3223
Escher, A., O'Kane, D.J., Lee, J., and Szalay, A.A. (1989)
Bacterial luciferase ı fusion protein is fully active as a monomer and highly sensitive in vivo to elevated temperature. PNAS, 86:6528-6532
Aguilar, O.M., Th"ny, B., Ramseier, Hennecke, H., Taormino, J., and Szalay, A.A. (1990)
Genes nifEN participating in FeMo cofactor biosynthesis and genes encoding dinitrogenase are part of the same operon in Bradyrhizobium species. Mol. Gen. Genet., 224:413-420
Koncz, C., Langridge, W.H.R., Olsson, O., Schell, J., and Szalay, A.A. (1990)
Bacterial and firefly luciferase genes in transgenic plants: advantages and disadvantages of a reporter gene system. Dev. Genetics 11:224-232
Escher, A., O'Kane, D.J., and Szalay, A.A. (1991)
The subunit polypeptide of bacterial luciferase determines light emission at 42oC. Mol. Gen. Genet. p230, 385-393
Langridge, W.H.R., Escher, A. Koncz, C., Schell, J. and Szalay, A.A. (1991)
Bacterial luciferase genes: A light emitting reporter system for in vivo measurement of gene expression. Technique, Journal of Methods in Cell and Molecular Biology. 3:3,91-98
Dale, P.L., B?ga, M., Szalay, A.A., Piatkowski, D., Salamini, F., and Bartels, D. (1992)
Expression of luciferase intransgenic tobacco mediated by a promoter element from desiccation tolerant Craterostigma plantagineum.Progress in Plant Growth Regulation. pp759-764.
Tamas, I.A., Langridge, W.H.R., Abel S.D., Crawford, S.W., Randall, J., Schell, J. Szalay, A.A. (1992)
Hormonal Control of apical dominance. Studies in tobacco transformed with bacterial luciferase and Agrobacterium rol genes. Progress in Plant Growth Regulation. pp418-430.
Chao J., Langridge W.H.R., and Szalay, A.A. 1992.
In vivo identification of developmentally regulated plant genes by T-DNA tagging with a promoterless luciferase gene fusion following by inverse polymerase chain reaction amplification. Plant Molecular Biology Reporter, Vol 10, no. 4, p354-361
Michel Detlef, Salamini Francesco, Bartels Dorothea, Dale Phyllis, Baga Monica, and Szalay Aladar (1993)
Analysis of a desiccation and ABA-responsive promoter isolated from the resurrection plant Craterostigma plantagineum. The Plant Journal Vol. 4, no. 1, 29-40
Pay Aniko, Jonak Claudia, B"gre Laszlo, Meskiene Irute, Mairanger Theresia, Szalay Aladar, Heberle-Bors Erwin and Hirt Hirbert (1993).
The Msk family alfalfa protein kinase genes encodes homologs of shaggy/glycogen synthase kinsase-3 and shows differential expression patterns in plant organs and development. The Plant Journal 3(6), 847-956
Escher, Alan and Szalay, Aladar A. (1993)
GroE-mediated folding of bacterial luciferase in vivo. Mol. Gen. Genet. 238:65-73
Wang, G., Mayerhofer, R., Langridge, W.H.R., and Szalay, A.A. (1993).
Expression of a Bacterial Luciferase Marker Gene in Bacillus Species. Proceedings of the VIIth Int. Symp. on Bio & Chemiluminescence. pp232-238
Langridge, W.H.R., Jiang, C., Wang, G., Giacoman, L., Dale, P., Baga, M., Fodor, I., and, Szalay, A.A. (1993)
A Luciferase Marker Gene System to Monitor Gene Expression in Bacteria, Plant and Virus Infected Animal Cells. Proceedings of the VIIth Int. Symp. on Bio & Chemiluminescence. pp222-226
Alton, G., Langridge, W.H.R. and Szalay A.A. (1993)
Evaluation of a Versitile Imaging Camera Apparatus for Chemiluminescent Elisa and Monitoring Bacterial Bioluminescence. Proceedings of the VIIth Int. Symp. on Bio & Chemiluminescence. pp3-7
Garcia Lloret, M.I., Guilbert, L.J., Langridge, W.H.R., Yui, J., T.G. Wegmann, T.G. and Szalay A.A. (l993)
Production of Reactive Oxygen Intermediates by Placental Trophoblasts. Proceedings of the VIIth Int. Symp. on Bio & Chemiluminescence. pp456-460
Langridge, W., Escher, A., Wang,G., Ayre, B., Fodor, I., and Szalay, A. (1994)
Low-Light Image Analysis of Transgenic Organisms using Bacterial Luciferase as a marker. J. Biolumin Chemilumin. 9:185-200
Mayerhofer R., Langridge W.H.R., Cormier M.J. & Szalay A.A. (1995)
Expression of Recombinant Renilla Luciferase in Transgenic Plants Results in High Levels of Light Emission, The Plant Journal 7:1031-1038
Lorenz, W.W., Cormier, M.J., O'Kane, D.J., Hua, D., Escher, A.A., Szalay, A.A. (1995)
Expression of the Renilla reniformis Luciferase Gene in Mammalian Cells, J. Biolumin Chemilumin; 11:31-7
Giacomin, L.T., Szalay, A.A. (1996)
Expression of a PALI promoter luciferase gene fusion in Arabidopsis thaliana in response to infection by phytopathogenic bacteria, Plant Science 116:59-72
Kereso, J., Praznovszky, T., Cserpan, I., Fodor, K., Katona, R., Csonka, E., Fatyol, K., Hollo, Gy., Szeles, A., Ross, A.R., Sumner, A.T., Szalay, A.A. & Hadlaczky, Gy. (1996) De novo chromosome formations by large-scale amplification of the centromeric region of mouse chromosomes, Chromosome Research, 4, 226-239
Hollo, Gy., Kereso, J., Praznovszky, T., Cserpan, I., Fodor, K., Katona, R., Csonka, E., Fatyol, K., Szeles, A., Szalay, A.A. & Hadlaczky, Gy. (1996)
Evidence for a megareplicon covering megabases of centromeric chromosome segments, Chromosome Research 4, 240-247
Mayerhofer, R., Haraki, K. and Szalay, A.A (1995)
Fish Monitoring of Spacial Expression of Firefly Luciferase in Transgenic Zebra Fish. J. Biolumin-Chemilumin. 10 (5):271-5
Wang, Y., Wang, G., O'Kane, D.J., Szalay, A.A. (1996)
The Renilla Luciferase-Modified GFP Fusion Protein is Functional in Transformed Cells," Proceedings of the IXth International Symposium on Bioluminescence and Chemiluminescence, Woods Hole, MA. pp. 419-422
Wang, G., Wang, Y., Cseh, S., and Szalay, A.A. (1996)
Imaging of Luciferase Fusion Gene Expression in Transformed Cells and Embryos, Proceedings of the IXth International Symposium on Bioluminescence and Chemiluminescence, Woods Hole, MA. pp. 415-418
Escher, A.P., Bagi, G., and Szalay, A.A. (1996)
"Genetic Study of Chaperonin-Bacterial Luciferase Interactions," Proceedings of the IXth International Symposium on Bioluminescence and Chemiluminescence, Woods Hole, MA. pp. 101-104
Langridge, W.H.R., Krausova, V.I., Szalay, A.A. & Fodor, I (1996)
Detection of baculovirus gene expression in insect cells and larvae by low light video image analysis, Journal of Virological Methods 61:151-156
In Press: Langridge, W.H.R., Szalay, A.A. (1996)
Bacterial and Coelenterate Luciferaes as Reporter Genes in Plant Cells, Methods in Molecular Biology Chong, D., Roberts, W., Arakawa, T., Illes, K., Bagi, G.,
Szalay, A.A., Slattery C.W., & Langridge, W.H.R, (1996)
Expression of the Human Milk Protein, -Casein in Transgenic Potato Plants, Transgenic Research
Cseh, S., Wang, G., Corselli. J., Nehlsen-Cannarella, S.L., Bailey, L.L., Szalay, A.A. (1996)
Rapid freezing of mouse embryos in ethylene glycol at different preimplantation stages, Acta Veterinaria Hungarica
Submitted: Cseh, S., Wang, G., Corselli. J., Nehlsen-Cannarella, S.L., Bailey, L.L., Szalay, A.A. (1996)
The effect of quick-freezing in ethylene glycol on the survival and in vitro developemnt of mouse embryos frozen at different preimplanation stages," Theriogenology
Diamond, D.C., Fagoaga, O.R., Nehlsen-Cannarella, S.L., Bailey, L.L., Szalay, A.A. (1996)
Sequence Comparison of Baboon ABO Histo-Blood Group Alleles: Lesions Found in O Alleles Differ Between Human and Baboon, PNAS