Symposium 1997: Abstract for G Zomer et al

Symposium 1997: Abstracts

Novel Acridane Esters as Efficient Chemiluminescence Substrates for the Detection of Horseradish Peroxidase Immunoassay Applications

Gijsbert Zomer, Marjorie Jacquemijns
National Institute of Public Health and the Environment, Bilthoven, The Netherlands

Recently, Akhavan-Tafti et al [1] reported the use of acridane carboxylate esters, e.g. 2,3,6-trifluorophenyl-N-methyl-acridane-9-carboxylate (PS-3) for the chemiluminescent (CL) detection of Horseradish Peroxidase (HRP) at sub attomole levels. We now would like to present novel aliphatic acridane esters (e.g. 1,1,1,3,3,3-hexafluoro-2-phenyl-isopropyl-N-methyl-2-chloroacridane- 9-carboxylate (GZ-8) and perfluoro-tert-butyl-N-methyl-2-chloroacridane-9-carboxylate (GZ-11)) with improved CL properties.

The preparation, CL properties and some applications of the new acridane esters will be presented.

[1] Akhavan-Tafti H, de Silva R, Arghavani Z, Eickholt RA, Handley RS, Schaap AP.
In: Bioluminescence and Chemiluminescence, Campbell AK, Kricka LJ, Stanley PE, editors. John Wiley & Sons, Chichester. 1994:199-203.