We have developed a new reporter system for rapid, non-invasive measurement of promoter/enhancer activity in intact mammalian cells (the CytoGem reporter system) based on the newly developed Emerald-Green and Topaz-Gold ultra-bright mutants of the Green Fluorescent Protein (GFP).

Our results show that reporter asays can easily be performed in many microplate formats, an that GFP expression is detected on the FluoroCount™ microplate fluorometer as early as 10 hours post-transfection in as little as 2,500 GFP-expressing cells. Reporter activity is linear over the entire range of the FluoroCount (4 orders of magnitude).

The response is robust, the signal being > 10 fold over basal levels for both systems. Additionally, both reporter systems work in all cell ty pes tested to date. Comparison with other commercially available GFP reporter systems showed that the pGFPemd [R] (Emerald-Green) system is the most sensitive GFP-based reporter system currently available.

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