Symposium 1997: Abstract for I Horneij et al

Symposium 1997: Abstracts

A Unique Chemiluminescent Substrate for High Throughput Screening Applications

Ineabel Horneij, Kelli Feather-Henigan, Paul D. Davis and Kimberly K. Hines

Pierce Chemical Company, 3747 N. Meridian Rd. Rockford, IL 61105, USA

Enhanced chemiluminescence has become useful for the rapid and sensitive detection of proteins and nucleic acids in both blotting and luminometer based applications.

A luminol based substrate, SuperSignal® LBA, with a novel enhancer has recently been developed for the detection of horseradish peroxidase (HRP). The system is easily adaptable to ELISAs that utilize colorimetric or other chemiluminescent substrates. When compared to other substrates in an Interleukin-2 (IL-2) ELISA, SuperSignal® LBA resulted in higher signal to noise ratios and increased sensitivity down to femtogram levels. When compared to 1,2-dioxetane which utilizes alkaline phosphatase and requires 20-30 minutes for maximum signal, SuperSignal® LBA reached a maximum signal within 1 minute and was 9 fold more sensitive.

SuperSignal® LBA also has the advantage of room temperature stability . This luminol based system offers the advantages of increased intensity, high signal to noise and immediate generation of light making it an ideal system for high throughput screening.